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Nanometer‐sectioning optical microscopy has become an indispensable tool in membrane‐related biomedical studies. Finally, many nanometer‐sectioning imaging schemes, such as variable‐angle total internal reflection fluorescence microscopy, metal‐induced energy transfer (MIET) imaging, and supercritical‐angle fluorescence microscopy have been introduced. However, these methods can measure a single layer...
The cell membrane is a dynamic and heterogeneous structure composed of distinct sub‐compartments. Within these compartments, preferential interactions occur among various lipids and proteins. Currently, it is still challenging to image these short‐lived membrane complexes, especially in living cells. In this work, we present a DNA‐based probe, termed “DNA Zipper”, which allows the membrane order and...
The cell membrane is a dynamic and heterogeneous structure composed of distinct sub‐compartments. Within these compartments, preferential interactions occur among various lipids and proteins. Currently, it is still challenging to image these short‐lived membrane complexes, especially in living cells. In this work, we present a DNA‐based probe, termed “DNA Zipper”, which allows the membrane order and...
This study presents a surface plasmon-enhanced total internal reflection fluorescence microscopy (TIRFM) and a surface plasmon polariton (SPP) phase microscopy techniques to image live cell membranes. In the surface plasmon-enhanced TIRFM, the developed microscopy technique is successfully applied to the real-time observation of the thrombomodulin proteins of live cell membranes.
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